Zebrafish HBAE3 Antibody / Hemoglobin Alpha Embryonic 3 Antibody (RZ1388)

Zebrafish HBAE3 Antibody Erythrocyte IHC. Immunohistochemical staining of paraffin-embedded zebrafish erythrocytes using Zebrafish HBAE3 Antibody / Hemoglobin Alpha Embryonic 3 Antibody demonstrates strong HRP-DAB brown staining within clustered erythroid cells. The staining pattern is consistent with expression of HBAE3, an embryonic alpha-globin protein that contributes to hemoglobin complex formation and oxygen transport during early vertebrate development. HBAE3 is associated with hematopoietic tissues, developing blood cell populations, and the embryonic vasculature, where it supports oxygen delivery to rapidly growing tissues. The observed erythrocyte-associated staining is consistent with the established role of HBAE3 in embryonic hematopoiesis, erythroid differentiation, and developmental oxygen transport. Heat-mediated antigen retrieval was performed in EDTA buffer, followed by overnight incubation with 2 µg/ml primary antibody and HRP-DAB visualization.

Zebrafish HBAE3 Antibody WB. Western blot analysis of zebrafish head tissue lysate (lane 1), whole female zebrafish tissue lysate (lane 2), whole male zebrafish tissue lysate (lane 3), and zebrafish embryo tissue lysate (lane 4) using Zebrafish HBAE3 Antibody / Hemoglobin Alpha Embryonic 3 Antibody demonstrates a distinct band at approximately 16 kDa, consistent with the expected molecular weight of HBAE3. Hemoglobin Alpha Embryonic 3 (HBAE3) is an embryonic alpha-globin protein that participates in hemoglobin complex formation and oxygen transport during early vertebrate development. HBAE3 is expressed in hematopoietic tissues, developing blood cell populations, and the embryonic vasculature, where it supports oxygen delivery to rapidly growing tissues. Detection of HBAE3 in zebrafish embryo lysate and additional tissue preparations is consistent with its established role in embryonic hematopoiesis, erythroid differentiation, and developmental oxygen transport. Electrophoresis was performed on a 12% SDS-PAGE gel under reducing conditions with 30 µg of protein loaded per lane, followed by nitrocellulose transfer and HRP-ECL detection.