HBZ Antibody / Hemoglobin zeta (FY12297)

Western blot analysis of HBZ using anti-HBZ antibody. Lane 1: rat bone tissue lysates, Lane 2: mouse bone tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBZ antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Expected molecular weight of Hemoglobin subunit zeta (HBZ) ~16 kDa (142 aa). On SDS-PAGE/Western blot, HBZ typically migrates at ~13–14 kDa due to its compact globin structure and atypical SDS binding. The observed band just below the 15 kDa marker is consistent with published reports for zeta-globin.