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banner Search results for Crk

Phospho-CrkL (Tyr207) Antibody / Crk like protein (clone 32C08)

Catalog No : FY12948
Applications : WB
Reactivity : Human
Format : Liquid
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All lanes use the Phospho-CrkL (Tyr207) antibody at 1:1K dilution for 1 hour at room temperature. A tight doublet is detected at ~40 kDa in K562 cells, running above the ~34 kDa predicted mass. The upward shift and doublet pattern are consistent with mono- and hyper-phosphorylated CrkL species, which migrate slower on SDS-PAGE than total CrkL.
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Crk Antibody

Catalog No : R31526
Applications : WB, IHC-P
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE human lung cancer tissue with Crk antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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CRK Antibody (pS41)

Catalog No : F40128
Applications : DB, ELISA
Reactivity : Human
Pred. Reactivity :Mouse, Rat
Format : Antigen affinity purified
Dot blot analysis of phospho-CRK antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted. P-Peptide=phos-peptide; NP-Peptide=nonphos-peptide.
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CrkL Antibody / Crk-like protein (clone 32C63)

Catalog No : FY12832
Applications : WB, IHC, ICC, IF, IP, FACS
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of CRKL using anti-CRKL antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRKL antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for CRKL at approximately 37 kDa. The expected molecular weight of CRKL is 34-39 kDa.
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CrkL Antibody / Crk-like protein

Catalog No : RQ7731
Applications : WB, IF, FACS, IP, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human U-87 MG cells with Crk-like protein antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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CrkL Antibody / Crk-like protein

Catalog No : R30906
Applications : WB, FACS
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Flow cytometry testing of fixed and permeabilized human U-251 cells with CrkL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CrkL antibody.
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CrkL Antibody / Crk-like protein

Catalog No : R34471
Applications : WB, IHC, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Mouse, Rat
Format : Antigen affinity purified
Western blot testing of K562 lysate with CrkL antibody at 0.01ug/ml. Expected molecular weight: 34~39kDa.
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Phospho-BCAR1 (Tyr410) Antibody / Breast cancer anti-estrogen resistance protein 1 (clone 32B03)

Catalog No : FY12440
Applications : WB
Reactivity : Human
Format : Liquid
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Western blot analysis of Phospho-BCAR1 (Y410) expression in HeLa treated with pervanadate cell lysate, using Phospho-BCAR1 (Tyr410) antibody.
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NEDD9 Antibody / hEF1 / CAS-L

Catalog No : R31702
Applications : WB, IHC-P, IF, FACS
Reactivity : Human, Mouse, Rat, Monkey
Format : Antigen affinity purified
IHC staining of FFPE human breast cancer tissue with NEDD9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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DOCK1 Antibody / Dedicator of cytokinesis protein 1

Catalog No : RQ7519
Applications : WB, IHC-P, FACS, IF, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue with DOCK1 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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