DOCK1 Antibody / Cell Migration Protein Antibody (RQ7519)

DOCK1 Antibody Esophageal Squamous Cell Carcinoma IF. Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue using DOCK1 Antibody / Cell Migration Protein Antibody demonstrates widespread cytoplasmic and membranous staining (red) throughout malignant epithelial cell populations, consistent with expression of Dedicator of Cytokinesis 1 (DOCK1). DOCK1 is a Rac guanine nucleotide exchange factor that regulates cell migration, cytoskeletal remodeling, and cellular adhesion through activation of Rac-dependent signaling pathways. The observed staining pattern is consistent with the established role of DOCK1 in coordinating dynamic cellular behavior and tissue organization. DAPI nuclear stain (blue) identifies cell nuclei and provides contrast to the predominantly cytoplasmic distribution of DOCK1. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

DOCK1 Antibody Human Intestinal Cancer IF. Immunofluorescent staining of FFPE human intestinal cancer tissue using DOCK1 Antibody / Cell Migration Protein Antibody demonstrates widespread cytoplasmic staining (red) throughout malignant epithelial and stromal-associated cellular compartments, consistent with expression of Dedicator of Cytokinesis 1 (DOCK1). DOCK1 is a Rac guanine nucleotide exchange factor that regulates cell migration, cytoskeletal remodeling, cellular adhesion, and signaling pathways involved in tissue organization. The extensive staining pattern is consistent with the established role of DOCK1 in coordinating dynamic cellular interactions and motility-associated processes within complex tissue microenvironments. DAPI nuclear stain (blue) identifies cell nuclei and provides contrast to the predominantly cytoplasmic distribution of DOCK1. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

DOCK1 Antibody Human Colorectal Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human colorectal adenocarcinoma tissue using DOCK1 Antibody / Cell Migration Protein Antibody demonstrates strong cytoplasmic and membranous staining within malignant gland-forming epithelial cells, consistent with expression of Dedicator of Cytokinesis 1 (DOCK1). DOCK1 is a Rac guanine nucleotide exchange factor that regulates cell migration, cytoskeletal remodeling, cellular adhesion, and tissue organization through activation of Rac-dependent signaling pathways. The observed staining pattern is consistent with the established role of DOCK1 in coordinating dynamic cellular behavior and interactions with the surrounding microenvironment. These findings support the utility of DOCK1 antibody for studies of cell migration, cytoskeletal regulation, and signaling pathways involved in tissue architecture and cellular motility. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

DOCK1 Antibody Human Lung Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human lung adenocarcinoma tissue using DOCK1 Antibody / Cell Migration Protein Antibody demonstrates moderate cytoplasmic staining within malignant epithelial cell populations, consistent with expression of Dedicator of Cytokinesis 1 (DOCK1). DOCK1 is a Rac guanine nucleotide exchange factor that regulates cell migration, cytoskeletal remodeling, cellular adhesion, and tissue organization through activation of Rac-dependent signaling pathways. The observed staining pattern is consistent with the established role of DOCK1 in coordinating dynamic cellular behavior and communication with the surrounding tissue microenvironment. These findings support the utility of DOCK1 antibody for studies of cell migration, cytoskeletal regulation, and signaling pathways involved in tissue architecture and cellular motility. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

DOCK1 Antibody Human Cell Line WB. Western blot analysis of human 293T and MCF7 cell lysates using DOCK1 Antibody / Cell Migration Protein Antibody demonstrates a distinct immunoreactive band at approximately 180-215 kDa, consistent with expression of Dedicator of Cytokinesis 1 (DOCK1). DOCK1, also known as DOCK180, is a Rac guanine nucleotide exchange factor that regulates cell migration, cytoskeletal remodeling, cellular adhesion, and tissue organization through activation of Rac-dependent signaling pathways. The observed expression across multiple human cell lines supports the utility of this antibody for investigating signaling networks that control cellular motility, morphogenesis, and dynamic cytoskeletal regulation. Predicted molecular weight: ~215 kDa; routinely observed at 180-215 kDa.

DOCK1 Antibody MCF7 Flow Cytometry. Flow cytometric analysis of human MCF7 cells using DOCK1 Antibody / Cell Migration Protein Antibody demonstrates a pronounced rightward shift of the blue histogram (DOCK1 antibody) relative to the green histogram (isotype control) and red histogram (cells alone), consistent with expression of Dedicator of Cytokinesis 1 (DOCK1). DOCK1 is a Rac guanine nucleotide exchange factor that regulates cell migration, cytoskeletal remodeling, cellular adhesion, and tissue organization through activation of Rac-dependent signaling pathways. The observed staining pattern supports detection of endogenous DOCK1 expression and demonstrates the utility of this antibody for studies of cellular motility, cytoskeletal regulation, and migration-associated signaling networks. Cells were stained with 1 ug antibody per million cells following blocking with goat sera.