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banner Search results for APOA1

APOA1 Antibody / Apolipoprotein A (clone 17G5)

Catalog No : FY12602
Applications : ELISA (Cap), WB
Reactivity : Human
Format : Lyophilized
Western blot analysis of APOA1 using anti-APOA1 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APOA1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith Tanon 5200 system. Western blot probed with anti-APOA1 shows a major band at ~25 kDa, lower than the predicted ~31 kDa, consistent with the known anomalous migration of the amphipathic APOA1 protein on SDS-PAGE.
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APOA1 Antibody / Apolipoprotein A I

Catalog No : R32382
Applications : WB, FACS, IHC-P, ELISA
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of human placenta lysate with APOA1 antibody. Predicted molecular weight ~30 kDa.
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APOA1 Antibody / Apolipoprotein A I

Catalog No : F50384
Applications : IHC, WB, ELISA
Reactivity : Human
Format : Purified
IHC analysis of FFPE human placenta tissue stained with APOA1 antibody
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APOA1 Antibody / Apolipoprotein A I

Catalog No : R32177
Applications : WB, IHC-P
Reactivity : Mouse
Format : Antigen affinity purified
Western blot testing of mouse liver lysate with Apolipoprotein A I antibody. Predicted molecular weight ~30 kDa.
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APOA1 Antibody / Apolipoprotein A I

Catalog No : R31886
Applications : WB, IHC-P
Reactivity : Rat
Format : Antigen affinity purified
Western blot testing of 1) rat liver and 2) rat testis lysate with Apolipoprotein A I antibody. Predicted molecular weight ~30 kDa.
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APOA1 Antibody / Apolipoprotein A I (clone APOA1/3661)

Catalog No : V8388
Applications : IHC-P
Reactivity : Human
Format : Purified
IHC staining of FFPE human liver with APOA1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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APOA1 Antibody / Apolipoprotein A I

Catalog No : R33650
Applications : WB, ELISA (peptide)
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of liver lysate with APOA1 antibody at 0.5ug/ml. Predicted molecular weight ~30kDa.
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APOA1BP Antibody

Catalog No : R35104
Applications : WB, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Cow, Dog, Mouse, Pig, Rat
Format : Antigen affinity purified
Western blot testing of human heart lysate with APOA1BP antibody at 0.5ug/ml. Predicted molecular weight: ~32kDa.
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APOA1 Antibody / Apolipoprotein A I

Catalog No : R35834
Applications : IHC, ELISA (peptide)
Reactivity : Human
Format : Antigen affinity purified
IHC testing of FFPE human liver with APOA1 antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
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NAXE Antibody / NADPHX epimerase

Catalog No : FY12412
Applications : WB, IHC, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of NAXE using anti-NAXE antibody. NAXE was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NAXE antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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