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Home >> Antibodies >> APOA1 Antibody

APOA1 Antibody (R32382)

  Catalog No Formulation Size Price (USD)  
Image R32382 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of human placenta lysate with APOA1 antibody. Predicted molecular weight ~30 kDa.
IHC testing of FFPE human liver cancer tissue with APOA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE human kidney cancer tissue with APOA1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Flow cytometry testing of human Caco-2 cells with APOA1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOA1 antibody.
Flow cytometry testing of human HepG2 cells with APOA1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOA1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P02647
Localization Cytoplasmic, membranous
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
ELISA : 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
Limitations This APOA1 antibody is available for research use only.
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Description

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL.

Application Notes

Optimal dilution of the APOA1 antibody should be determined by the researcher.

Immunogen

Amino acids D25-Q267 of the human protein were used as the immunogen for the APOA1 antibody.

Storage

After reconstitution, the APOA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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