APOA1 Antibody APOA1/3661 / ApoA-I Antibody [clone APOA1/3661] (V8388)

APOA1 Antibody APOA1/3661 / ApoA-I Antibody in Human Liver IHC. Immunohistochemistry staining of FFPE human liver tissue with APOA1 Antibody clone APOA1/3661 shows granular cytoplasmic staining within hepatocytes, with variable staining intensity across the hepatic parenchyma. The pattern is consistent with intracellular ApoA-I associated with its synthesis and secretion by hepatocytes. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing.

APOA1 Antibody APOA1/3661 in Human Hepatocytes IHC. Immunohistochemistry staining of FFPE human liver tissue with APOA1 Antibody clone APOA1/3661 shows heterogeneous granular cytoplasmic staining of hepatocytes, with strongly positive cells interspersed among more weakly stained cells. Nuclear staining is not prominent, consistent with the secreted nature of Apolipoprotein A-I. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing.

Purified APOA1 Antibody APOA1/3661 SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free APOA1 Antibody clone APOA1/3661 under reduced (R) and non-reduced (NR) conditions demonstrates the integrity of the mouse monoclonal antibody preparation. Under reducing conditions, prominent immunoglobulin heavy- and light-chain bands are visible at approximately 50 kDa and 25 kDa, respectively, while the intact non-reduced antibody migrates predominantly near 150 kDa.