NSJ Bioreagents

KRT13 antibody is used to identify and study KRT13, also known as Keratin 13 and Cytokeratin 13, a structural protein found in the skin, specifically in the outer layer known as the epidermis. This protein is responsible for providing strength and stability to the skin cells, ensuring that the skin remains intact and able to withstand external stressors. One of the key functions of KRT13 is to protect the skin from damage caused by UV rays, pollution, and other environmental factors. By forming a barrier on the skin's surface, KRT13 helps prevent water loss and protects against harmful substances that can cause skin irritation and inflammation. KRT13 plays a critical role in the process of skin regeneration. When the skin is damaged, Keratin 13 helps to repair and rebuild the skin cells, promoting healing and overall skin health. Without an adequate amount of Keratin 13, the skin may be more susceptible to damage and aging.

A KRT13 antibody can be used in various research techniques such as western blot, flow cytometry, immunofluorescence, immunohistochemistry, and ELISA, to study this important protein.

Western blot, a commonly used technique in molecular biology, utilizes a KRT13 antibody to detect the presence of KRT13 protein in cell lysates. By visualizing the specific bands on the blot, researchers can determine the expression levels of Keratin 13 and study its role in various cellular processes.

Flow cytometry is another powerful tool that employs a KRT13 antibody to analyze the expression of KRT13 on a single-cell level. By staining cells with KRT13 antibody and analyzing them using flow cytometry, researchers can gain insights into the distribution and abundance of KRT13 in different cell populations.

Immunofluorescence and immunohistochemistry are techniques that take advantage of the specific binding capabilities of a KRT13 antibody to visualize the localization of KRT13 within cells or tissue samples. By using fluorescently labeled KRT13 antibody, researchers can observe the precise subcellular localization of KRT13 and study its dynamic distribution in different cellular conditions.

ELISA, or enzyme-linked immunosorbent assay, is a sensitive technique that utilizes a KRT13 antibody to quantify the levels of KRT13 protein in biological samples. By detecting the signal produced by the interaction between KRT13 antibody and KRT13 protein, researchers can measure the concentration of Keratin 13 with high precision and accuracy.

The versatility and specificity of KRT13 antibody make it a valuable tool for researchers in the fields of cell biology, molecular biology, and beyond. Through techniques such as western blot, flow cytometry, immunofluorescence, immunohistochemistry, and ELISA, researchers can use the KRT13 antibody to uncover the mysteries of KRT13 protein and its role in various biological processes.