KRT13 Antibody rKRT13/9623 / Keratin 13 Monoclonal Antibody [clone rKRT13/9623] (V5478)

KRT13 Antibody rKRT13/9623 by IHC in Human Prostate Carcinoma. Immunohistochemistry of FFPE human prostate carcinoma tissue using KRT13 Antibody rKRT13/9623. Brown immunoreactivity is observed in epithelial structures, with staining concentrated in the cytoplasm of cells surrounding glandular spaces. Staining intensity varies across the tissue section, while nuclei are counterstained blue with hematoxylin. The inset shows the negative control, prepared by substituting PBS for the primary antibody, with minimal brown staining. The Keratin 13 Monoclonal Antibody demonstrates localized epithelial immunoreactivity. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

KRT13 Antibody rKRT13/9623 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free KRT13 Antibody rKRT13/9623 under non-reducing (NR) and reducing (R) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating near 150 kDa, consistent with intact immunoglobulin. Under reducing conditions, a prominent band is visible near 50 kDa, corresponding to the immunoglobulin heavy chain. The expected light-chain band near 25 kDa is not clearly resolved in the displayed image. The Keratin 13 Monoclonal Antibody exhibits the principal electrophoretic features of a purified mouse monoclonal antibody preparation.