Cytokeratin 13 Antibody KRT13/2659 / CK13 Monoclonal Antibody [clone KRT13/2659] (V7916)

Cytokeratin 13 Antibody KRT13/2659 by FACS in Human HeLa Cells. Flow cytometry analysis of permeabilized human HeLa cells using Cytokeratin 13 Antibody KRT13/2659. The blue histogram representing antibody staining shows a rightward fluorescence shift relative to the red isotype control histogram. The two distributions are separated, with antibody-treated cells exhibiting increased fluorescence intensity. The CK13 Monoclonal Antibody demonstrates immunoreactivity in permeabilized HeLa cells under the tested conditions.

Cytokeratin 13 Antibody KRT13/2659 by IHC in Human Prostate Carcinoma. Immunohistochemistry of FFPE human prostate carcinoma tissue using Cytokeratin 13 Antibody KRT13/2659. Brown immunoreactivity is visible in epithelial structures, with prominent staining along the cytoplasmic regions of cells surrounding glandular spaces. Staining intensity varies across the tissue section, while nuclei are counterstained blue with hematoxylin. The CK13 Monoclonal Antibody demonstrates an epithelial staining pattern in this specimen. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

Cytokeratin 13 Antibody KRT13/2659 by IHC in Human Tonsil. Immunohistochemistry of FFPE human tonsil tissue using Cytokeratin 13 Antibody KRT13/2659. Brown cytoplasmic staining is concentrated in the stratified squamous epithelial compartment, contrasting with the predominantly unstained lymphoid tissue. The CK13 Monoclonal Antibody highlights the epithelial distribution of keratin 13, consistent with its association with nonkeratinized squamous epithelial differentiation. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

Cytokeratin 13 Antibody KRT13/2659 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Cytokeratin 13 Antibody KRT13/2659 under non-reducing (NR) and reducing (R) conditions. The non-reducing lane contains a prominent band migrating at approximately 150 kDa, consistent with intact immunoglobulin. The reducing lane shows a distinct band near 25 kDa, corresponding to the immunoglobulin light chain. The CK13 Monoclonal Antibody exhibits an electrophoretic profile consistent with a purified mouse monoclonal antibody preparation.