NSJ Bioreagents

AQP4 (Aquaporin-4) is a water channel protein that plays a crucial role in the regulation of water homeostasis in the central nervous system. Dysregulation of AQP4 has been linked to various neurological disorders, making it a key target for research and diagnostic purposes. By utilizing an AQP4 antibody in techniques such as western blot, immunohistochemistry, immunofluorescence, and flow cytometry, researchers can gain valuable insights into the expression patterns, localization, and regulation of AQP4 in health and disease.

Western blotting is a common technique used to detect proteins in a sample. In the case of AQP4 detection, a specific AQP4 antibody is used to detect the presence of the protein in a cell lysate. This technique allows for the quantification of AQP4 protein levels in different tissues or cell types, providing valuable insight into its expression patterns and potential dysregulation in disease states.

Immunohistochemistry is a technique used to visualize the localization of proteins within tissues. By using a specific AQP4 antibody, researchers can stain tissue sections to observe the distribution of AQP4 protein in different regions of the central nervous system. This technique can provide valuable information about the cellular localization of AQP4 and its potential role in various neurological disorders.

Immunofluorescence is another imaging technique that allows for the visualization of proteins within cells or tissues. By using a fluorescently labeled AQP4 antibody, researchers can observe the spatial distribution of AQP4 protein in real-time. This technique is particularly useful for studying the dynamic localization of AQP4 in response to different stimuli or disease conditions.

Flow cytometry is a high-throughput technique used to analyze the expression levels of proteins on a single-cell basis. By using a specific AQP4 antibody conjugated to fluorescent dyes, researchers can quantify the expression of AQP4 on individual cells within a heterogeneous population. This technique is valuable for studying the heterogeneity of AQP4 expression in different cell types and its potential implications in disease progression.

The specific detection of AQP4 protein using AQP4 antibodies is essential for understanding its role in various neurological disorders.