AQP-4 Antibody / Mercurial-Insensitive Water Channel Antibody (RQ7058)

AQP-4 Antibody Staining in Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue using AQP-4 Antibody shows brown labeling distributed throughout the neural tissue, including fine cellular processes and vascular-associated structures. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The staining pattern demonstrates detection of AQP4 in mouse brain sections using this Mercurial-Insensitive Water Channel Antibody.

AQP-4 Antibody Staining in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue using AQP-4 Antibody shows distinct brown labeling of fine processes and vascular-associated structures throughout the section. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The distribution is consistent with the characteristic enrichment of AQP4 in astrocytic processes and demonstrates rat brain tissue detection with this Mercurial-Insensitive Water Channel Antibody.

AQP-4 Antibody Detection in Human, Rat and Mouse Lysates WB. Western blot testing using AQP-4 Antibody shows immunoreactive bands within the expected approximately 35-45 kDa range for AQP4. Lane 1: human U-87 MG glioblastoma cell lysate; lane 2: rat brain tissue lysate; lane 3: mouse brain tissue lysate. The rat brain sample produces the strongest signal, while discrete bands are also visible in the human and mouse samples. Differences in apparent molecular weight can reflect AQP4 glycosylation. The three-species result demonstrates detection using this Mercurial-Insensitive Water Channel Antibody.

AQP-4 Antibody Detection in Human A431 Epidermoid Carcinoma Cells FACS. Flow cytometry analysis of human A431 epidermoid carcinoma cells using AQP-4 Antibody at 1 ug/million cells shows a clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green) and cells-alone control (red). Cells were blocked with goat sera before staining. The separation of the blue population from both controls demonstrates detection of AQP4 in A431 cells using this Mercurial-Insensitive Water Channel Antibody.