AQP4 Antibody / Aquaporin 4 Polyclonal Antibody (R32145)

AQP4 Antibody Staining in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue using AQP4 Antibody shows distinct brown staining associated with epithelial/tumor structures, with stronger signal along portions of the cell periphery and surrounding tissue architecture. HIER was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining. This tissue-based staining demonstrates detection with the Aquaporin 4 Polyclonal Antibody in human FFPE sections.

AQP4 Antibody Detection in Rat and Mouse Brain WB. Western blot testing of rat and mouse brain tissue lysates using AQP4 Antibody shows a strong predominant band at approximately 35-40 kDa in all four lanes. Lanes 1-2 contain rat brain and lanes 3-4 contain mouse brain. Additional higher molecular weight signal is visible, particularly in lane 3. AQP4 is known to migrate within an approximately 35-45 kDa range depending on glycosylation. The consistent major band across both species demonstrates detection of AQP4 with this Aquaporin 4 Polyclonal Antibody.

AQP4 Antibody Staining in FFPE Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain using AQP4 Antibody shows widespread brown signal with a fine membranous and process-associated pattern throughout the neural tissue. HIER was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining. The distribution is consistent with the characteristic localization of AQP4 within astrocytic processes and demonstrates tissue detection using this Aquaporin 4 Polyclonal Antibody.

AQP4 Antibody Staining in Frozen Mouse Brain IHC. Immunohistochemistry staining of frozen mouse brain using AQP4 Antibody shows discrete dark brown labeling of elongated vascular-associated structures and fine cellular processes against relatively low background. The prominent vessel-associated pattern is consistent with enrichment of AQP4 at astrocytic endfeet surrounding the cerebral microvasculature. This frozen-tissue result complements the FFPE mouse brain staining obtained with the Aquaporin 4 Polyclonal Antibody.

AQP4 Antibody Staining in FFPE Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain using AQP4 Antibody shows distinct brown labeling of vascular-associated structures and surrounding neural tissue. Particularly prominent staining is visible along small vessels and fine processes, consistent with the characteristic perivascular distribution of AQP4 in astrocytic endfeet. HIER was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining. This pattern demonstrates rat brain tissue detection using the Aquaporin 4 Polyclonal Antibody.

AQP4 Antibody Staining in Frozen Rat Brain IHC. Immunohistochemistry staining of frozen rat brain using AQP4 Antibody shows strong, sharply defined labeling of vascular-associated structures and fine processes within the surrounding neural tissue. The prominent perivascular pattern is consistent with enrichment of AQP4 at astrocytic endfeet associated with the cerebral microvasculature. The staining provides complementary frozen-tissue evidence for detection of rat AQP4 using this Aquaporin 4 Polyclonal Antibody