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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2461 to 2470
    PMF1 Antibody / Polyamine modulated factor 1 (clone 30P63)
    Catalog No : FY13197
    Applications : WB, IP
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of PMF1 expression in (1) human HeLa cell lysate; (2) mouse RAW264.7 cell lysate using PMF1 antibody. Predicted molecular weight ~23 kDa.
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    Clusterin Antibody / Clu (clone 30C68)
    Catalog No : FY13203
    Applications : WB, IHC, IP
    Reactivity : Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Immunoprecipitation analysis using the Clu antibody at 1:50 dilution. Western blot at 1:500 dilution. Clusterin IP from HeLa lysate. Under reducing conditions, a faint clusterin band appears in the low-40 kDa range (alpha chain). Prominent ~50-55 kDa and ~25 kDa bands represent IgG heavy and light chains from the IP antibody.
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    Histone H3 (mono methyl K79) Antibody / HIST1H3A (clone 31H92)
    Catalog No : FY13214
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the Histone H3 (mono methyl K79) antibody at 1:1000 dilution for 1 hour at room temperature. Expected molecular weight ~15 kDa.
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    TAGAP Antibody / T cell activation Rho GTPase activating protein (clone 30T40)
    Catalog No : FY13222
    Applications : WB, IP
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of TAGAP using anti-TAGAP antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGAP antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot detection of TAGAP shows a single band at ~70 kDa across tested lysates. Despite a calculated mass of ~81 kDa, TAGAP frequently migrates lower on SDS-PAGE, consistent with isoform- and composition-dependent mobility.
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    Histone H2A (mono methyl K118) Antibody / HIST1H2AB (clone 31H85)
    Catalog No : FY13228
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the Histone H2A (mono methyl K118) antibody at 1:5000 dilution for 1 hour at room temperature. Predicted molecular weight ~15 kDa.
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    SLC27A4 Antibody / Solute carrier family 27 member 4 (clone 30S71)
    Catalog No : FY13230
    Applications : WB, IHC, ICC, IF, IP
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Immunoprecipitation analysis using the antibody at 1:50 dilution. Western blot at 1:1000 dilution. Immunoprecipitation of SLC27A4 (FATP4) from HeLa lysate followed by Western blot detection shows a specific band at ~60 kDa, also present in the input sample and absent in the negative control. A separate Western blot of total lysate detects the expected ~72 kDa full-length protein. SLC27A4 is not known to be glycosylated, and its predicted mass (~72 kDa) reflects the complete 643-amino-acid translation product. The lower-migrating ~60 kDa form likely represents a truncated or processed species of SLC27A4 rather than a deglycosylated variant.
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    Phospho-ULK1 (pSer556) Antibody / Unc-51 like autophagy activating kinase 1 (clone 31U30)
    Catalog No : FY13231
    Applications : IF, ICC, WB
    Reactivity : Human, Mouse
    Format : Liquid
    Recrabbitmono
    Immunofluorescent analysis using the Phospho-ULK1 (pSer556) antibody (green) at 1:50 dilution.
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    PSMB10 Antibody / Proteasome subunit beta type 10 (clone 30P25)
    Catalog No : FY13232
    Applications : WB, IHC
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of PSMB10 expression in human Raji cell lysate. Predicted molecular weight ~29 kDa.
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    PDIA4 Antibody / Protein disulfide-isomerase A4 / ERp72 (clone 32P79)
    Catalog No : FY13237
    Applications : WB, IHC
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the PDIA4 antibody at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight ~73 kDa.
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    Phospho-Beta Arrestin 1 (pSer412) Antibody / ARRB1 (clone 32A60)
    Catalog No : FY13239
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the Phospho-Beta Arrestin 1 (pSer412) antibody at 1:1000 dilution for 1 hour at room temperature. Western blot analysis of phospho-beta-arrestin 1 (pSer412) shows a dominant band at ~55 kDa and a secondary band near ~37 kDa across multiple cell lines. Although the predicted size of beta-arrestin 1 is ~47 kDa, phosphorylation at Ser412 causes an upward mobility shift, while the lower band likely represents a truncated or partially degraded form of the protein.
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