CASP3 Antibody / Caspase 3 [clone 30C96] (FY13246)

Western blot analysis of Caspase 3 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate; (3) rat brain lysate using CASP3 antibody. The predicted molecular weight of Caspase 3 is at ~32 kDa.

Western blot analysis of Caspase 3 using anti-CASP3 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human 293T whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 3 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot of caspase 3 in cells treated ± staurosporine (STS). In untreated samples a ~32 kDa band corresponding to pro-caspase-3 is detected. STS treatment reduces the proform and generates a ~17 kDa band, consistent with the cleaved large subunit of active caspase-3 (p17). This shift indicates caspase-3 activation by STS.

Immunohistochemical staining of Caspase 3 using anti-CASP3 antibody. Caspase 3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CASP3 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

Immunohistochemical staining of Caspase 3 using anti-CASP3 antibody. Caspase 3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CASP3 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.