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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2441 to 2450
    SOCS7 Antibody / Suppressor of cytokine signaling 7 (clone 30S97)
    Catalog No : FY13089
    Applications : WB, IHC
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of SOCS7 using anti-SOCS7 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS7 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A band is detected in the commonly cited 58-63 kDa range. This migration is consistent with endogenous SOCS7 and reflects differences in phosphorylation state and isoform usage, which modestly alter SDS-PAGE mobility.
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    ACBD3 Antibody / Acyl-CoA binding domain containing protein 3 (clone 32A12)
    Catalog No : FY13092
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    All lanes use the ACBD3 antibody at 1:1K dilution for 1 hour at room temperature. A single major band is detected just below the 70 kDa marker, consistent with the expected migration of full-length ACBD3, which typically runs above its predicted 61 kDa mass due to phosphorylation and the structured coiled-coil/GOLD domains that alter SDS-PAGE mobility. The observed band therefore represents the mature, Golgi-associated form of ACBD3.
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    Phospho-Syk (pTyr323) Antibody / SYK (clone 32S11)
    Catalog No : FY13093
    Applications : WB, IHC, ICC, IF
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    FRG1 Antibody / FSHD region gene 1 (clone 32F07)
    Catalog No : FY13100
    Applications : WB, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of rat brain tissue lysate using the FRG1 antibody at 1:3000 dilution for 1 hour at room temperature. FRG1 antibody detects a single band at ~33-35 kDa. Although the calculated mass of FRG1 is ~29 kDa, published work and reagent data frequently show an apparent size in the low-to-mid 30 kDa range, likely reflecting phosphorylation-dependent mobility and nuclear enrichment.
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    DAZAP1 Antibody / DAZ associated protein 1 (clone 29D87)
    Catalog No : FY13105
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of DAZAP1 expression in human Jurkat cell lysate using DAZAP1 antibody. Predicted molecular weight ~43 kDa.
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    GAS2 Antibody / Growth arrest specific protein 2 (clone 31G23)
    Catalog No : FY13112
    Applications : WB, IHC
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of GAS2 expression in human Jurkat cell lysate using GAS2 antibody. Predicted molecular weight ~35 kDa.
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    TIM50 Antibody / Translocase of inner mitochondrial membrane 50 (clone 32T72)
    Catalog No : FY13126
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of mouse and rat samples using the TIM50 antibody at 1:1000 dilution for 1 hour at room temperature. TIM50 antibody detects a consistent band at ~36-37 kDa across multiple samples. Although the calculated mass is ~40 kDa, TIM50 undergoes mitochondrial presequence cleavage upon import, yielding a mature ~36 kDa form that predominates in western blot analyses of endogenous samples.
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    K27-linkage Specific Ubiquitin Antibody / UBB / UBC (clone 31U80)
    Catalog No : FY13127
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of K27-linkage Specific Ubiquitin expression in human HeLa cell lysate using K27-linkage Specific Ubiquitin antibody. A strong band near ~20 kDa in HeLa lysate is detected, corresponding to K27-linked di-ubiquitin. Although the UBB and UBC precursors have predicted masses of ~26 kDa and ~77 kDa, they are rapidly processed into monomeric and short polyubiquitin chains in cells; K27-linked Ub2 species are therefore the predominant form detected under standard conditions.
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    SYVN1 Antibody / Synoviolin / HRD1 (clone 32S38)
    Catalog No : FY13144
    Applications : WB, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of HRD1/SYVN1 using anti-SYVN1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYVN1 antibody at 1: 500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of HRD1/SYVN1 is at 68 kDa.
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    Phospho-eIF4EBP1 (pThr70) Antibody / EIF4EBP1 (clone 32E48)
    Catalog No : FY13152
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Phospho-eIF4EBP1 using anti-Phospho-eIF4EBP1 (pThr70) antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-eIF4EBP1 (Thr70) antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of Phospho-eIF4EBP1 is ~17 kDa.
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