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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 1211 to 1220
    Synaptophysin Antibody (clone SYP/4389R)
    Catalog No : V9165
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Microvalidated
    Recrabbitmono
    Synaptophysin Antibody Microarray Specificity Validated Clone SYP/4389R. Protein microarray specificity validation using the HuProt(TM) human protein microarray platform containing more than 19,000 full-length human proteins demonstrates selective recognition of the Synaptophysin / SYP target by monoclonal antibody clone SYP/4389R. Signal ranking analysis identifies SYP as the top-ranked protein on the array with a strong Z-score signal compared with other proteins tested, indicating preferential binding of the antibody to Synaptophysin. Additional proteins such as CD47 and FBXO2 show substantially lower signal intensity, further supporting the high specificity of this microarray specificity validated antibody. In HuProt(TM) microarray analysis, the Z-score represents the strength of antibody binding signal relative to the overall signal distribution on the array, expressed as standard deviations above the mean signal intensity. Targets are ranked according to their Z-scores, and the S-score represents the difference between consecutive Z-scores in the ranked list. The S-score therefore reflects the relative target specificity of the antibody for its intended antigen. The strong enrichment of the Synaptophysin signal and the clear separation from subsequent ranked proteins demonstrate the specificity of Synaptophysin Antibody Microarray Specificity Validated Clone SYP/4389R for the Synaptophysin protein during large-scale protein microarray screening.
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    CDH1 Antibody / E-Cadherin (clone CDH1/7034R)
    Catalog No : V9169
    Applications : IHC-P, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CDH1 Antibody Human Colon IHC. Immunohistochemistry analysis of Cadherin 1 / CDH1 expression in FFPE human colon tissue using clone CDH1/7034R antibody, showing strong membranous HRP-DAB brown staining in epithelial cells lining glandular structures with clear cell-cell junction localization, while surrounding stromal tissue remains largely negative. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing. Signal highlights epithelial organization and regulated cell adhesion within colon mucosa.
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    Prolactin Antibody / PRL (clone PRL/4908R)
    Catalog No : V9172
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human pituitary tissue with Prolactin antibody (clone PRL/4908R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    CD38 Antibody (clone CD38/6448R)
    Catalog No : V9180
    Applications : WB, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    CD38 Antibody human prostate adenocarcinoma tissue IHC. Immunohistochemistry analysis of CD38 expression in FFPE human prostate adenocarcinoma tissue using CD38 antibody clone CD38/6448R at 2 ug/ml. Strong membranous and cytoplasmic HRP-DAB brown staining highlights tumor-infiltrating lymphocytes within stromal and peri-glandular regions, consistent with an activated lymphocyte marker pattern, with positive immune cells interspersed among malignant epithelial structures. The staining demonstrates immune activation within the tumor microenvironment, while tumor cells show variable staining. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to antibody incubation.
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    CD33 Antibody (clone SIGLEC3/7046R)
    Catalog No : V9181
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    SDS-PAGE analysis of purified, BSA-free recombinant CD33 antibody (clone SIGLEC3/7046R) as confirmation of integrity and purity.
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    MSH6 Antibody (clone MSH6/6654R)
    Catalog No : V9182
    Applications : ELISA, WB, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human colon carcinoma tissue with recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    Albumin Antibody / ALB (clone ALB/6411R)
    Catalog No : V9185
    Applications : ELISA, IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human liver carcinoma in colon tissue with ALB antibody (clone ALB/6411R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    TMEPAI Antibody / PMEPA1 (clone PMEPA1/6421R)
    Catalog No : V9209
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human prostate carcinoma tissue with recombinant TMEPA1 antibody (clone PMEPA1/6421R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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    His Tag Antibody / Histidine (clone 6HIS/6402R)
    Catalog No : V9221
    Applications : WB, FACS, IF
    Reactivity : Species independent
    Format : Purified
    Recrabbitmono
    Western blot analysis of Poly His antibody in His-tagged protein samples. A recombinant His-tagged protein was resolved by SDS-PAGE under reducing conditions and probed with the recombinant rabbit monoclonal Poly His antibody clone 6HIS/6402R. A strong immunoreactive band is observed at approximately 20 kDa, consistent with the expected molecular weight of the His-tagged target protein. The clean banding pattern supports specific detection of polyhistidine-tagged recombinant proteins by clone 6HIS/6402R.
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    Mammaglobin A Antibody / SCGB2A2 (clone MGB/4811R)
    Catalog No : V9243
    Applications : IHC-P
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    MGB1 Antibody clone MGB/4811R immunohistochemistry of human lactating breast tissue. FFPE human lactating breast tissue was stained with MGB1 antibody (clone MGB/4811R). HRP-DAB brown chromogenic signal highlights strong cytoplasmic staining in secretory epithelial cells lining mammary glandular structures and luminal secretions, consistent with high expression of Mammaglobin A / SCGB2A2 in lactating breast epithelium, while surrounding stromal cells show minimal staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.
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