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Phospho Antibody / Antibodies

Home >> Antibodies >> Phospho Antibody / Antibodies

Phospho Antibody / Antibodies

Phospho antibodies available from NSJ Bioreagents:
Found all antibodies, displaying 301 to 310
Phospho-Syk (pTyr323) Antibody / SYK (clone 32S11)
Catalog No : FY13093
Applications : WB, IHC, ICC, IF
Reactivity : Human
Format : Liquid
Recrabbitmono
Phospho-eIF4EBP1 (pThr70) Antibody / EIF4EBP1 (clone 32E48)
Catalog No : FY13152
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-eIF4EBP1 using anti-Phospho-eIF4EBP1 (pThr70) antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-eIF4EBP1 (Thr70) antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of Phospho-eIF4EBP1 is ~17 kDa.
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Phospho-JAK1 (pTyr1034/pTyr1035) Antibody (clone 31J16)
Catalog No : FY13168
Applications : WB, IHC
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot testing of lysate from human Ramos cells treated with pervanadate, using Phospho-JAK1 (TpTyr1034/pTyr1035) antibody. Phospho-JAK1 (pTyr1034/pTyr1035) detected in pervanadate-treated Ramos lysate as a single band at ~125–130 kDa, consistent with activated full-length JAK1.
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Phospho-ULK1 (pSer556) Antibody / Unc-51 like autophagy activating kinase 1 (clone 31U30)
Catalog No : FY13231
Applications : IF, ICC, WB
Reactivity : Human, Mouse
Format : Liquid
Recrabbitmono
Immunofluorescent analysis using the Phospho-ULK1 (pSer556) antibody (green) at 1:50 dilution.
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Phospho-Beta Arrestin 1 (pSer412) Antibody / ARRB1 (clone 32A60)
Catalog No : FY13239
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Western blot testing of human samples using the Phospho-Beta Arrestin 1 (pSer412) antibody at 1:1000 dilution for 1 hour at room temperature. Western blot analysis of phospho-beta-arrestin 1 (pSer412) shows a dominant band at ~55 kDa and a secondary band near ~37 kDa across multiple cell lines. Although the predicted size of beta-arrestin 1 is ~47 kDa, phosphorylation at Ser412 causes an upward mobility shift, while the lower band likely represents a truncated or partially degraded form of the protein.
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Phospho-Caveolin 1 (pTyr14) Antibody / CAV1 (clone 32C49)
Catalog No : FY13264
Applications : WB
Reactivity : Human
Format : Liquid
Recrabbitmono
Phospho-PKA C (pThr197) Antibody / PRKACA/PRKACB/PRKACG (clone 32P41)
Catalog No : FY13272
Applications : WB, IHC
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
Western blot analysis of P-PRKA/C/G using anti-Phospho-PKA C (pThr197) antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-PKA C (Thr197) antibody at 1:500 overnight at 4oC, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The predicted molecular weight of P-PRKA/C/G is ~40 kDa.
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Phospho-Rb (pSer780) Antibody / Retinoblastoma (clone 31R44)
Catalog No : FY13298
Applications : WB
Reactivity : Human, Mouse
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-Rb (pS780) expression in human K562 cell lysate using Phospho-Rb (pSer780) antibody. Predicted molecular weight ~106 kDa.
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Phospho-GLUT4 (pSer488) Antibody / SLC2A4 (clone 32S34)
Catalog No : FY13345
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Liquid
Recrabbitmono
All lanes use the Phospho-GLUT4 (pSer488) antibody at 1:1000 dilution for 1 hour at room temperature. Predicted molecular weight ~55 kDa and ~44 kDa (two isoforms).
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Phospho-RIP3 (pSer232) Antibody / RIPK3 (clone 31R36)
Catalog No : FY13346
Applications : WB
Reactivity : Mouse, Rat
Format : Liquid
Recrabbitmono
Western blot analysis of Phospho-RIP3 (S232) using anti-Phospho-RIP3 (pS232) antibody. Lane 1: rat spleen tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-RIP3 (S232) antibody (S232) at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of Phospho-RIP3 (S232) is at 50 kDa.
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