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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Major Vault Protein Antibody for IF / MVP Immunofluorescence Antibody [clone 1014] (V3042)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V3042-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V3042-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V3042SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | 1014 |
| Purity | Protein G affinity chromatography |
| UniProt | Q14764 |
| Localization | Cytoplasmic, nuclear |
| Applications | Western Blot : 1-2ug/ml Immunofluorescence : 1-2ug/ml |
| Limitations | This Major Vault Protein Antibody for IF / MVP Immunofluorescence Antibody is available for research use only. |
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Major Vault Protein (MVP), encoded by the MVP gene, is the primary structural component of vault ribonucleoprotein particles, large cytoplasmic complexes involved in intracellular transport, signal transduction, and cellular stress responses. MVP is also widely known as Lung resistance-related protein (LRP), reflecting its strong association with multidrug resistance in cancer. MVP is broadly expressed across tissues, with prominent expression in epithelial cells, immune cells, and tumor populations exhibiting therapy-resistant phenotypes.
Major Vault Protein Antibody for IF, also referred to as MVP immunofluorescence antibody or LRP antibody for immunofluorescence in the literature, is specifically suited for high-resolution visualization of MVP localization within intact cells. This Major Vault Protein Antibody for IF / MVP Immunofluorescence Antibody is uniquely positioned for studies requiring spatial mapping of protein distribution, intracellular trafficking, and dynamic relocalization under different biological conditions. Clone 1014 antibody provides consistent signal detection for imaging applications, and this mouse monoclonal antibody has been utilized in peer-reviewed studies, supporting its relevance in fluorescence-based research.
In immunofluorescence applications, MVP is predominantly detected in the cytoplasm with a characteristic diffuse to punctate staining pattern that reflects vault particle distribution. Perinuclear enrichment is frequently observed, consistent with MVP association with vesicular transport pathways and cytoskeletal networks. These patterns are especially well resolved by immunofluorescence, allowing visualization of vault particle organization that is not accessible through bulk protein detection methods such as western blot.
A key advantage of using an MVP antibody for IF is the ability to monitor changes in protein localization in response to cellular stress, drug exposure, or signaling activation. MVP has been shown to redistribute within the cell under conditions associated with chemotherapeutic treatment and oxidative stress, and immunofluorescence enables direct observation of these spatial changes. This makes MVP immunofluorescence antibody particularly valuable for studying mechanisms of drug resistance at the single-cell level, where subcellular localization may correlate with functional activity.
MVP plays a central role in vault complex assembly, forming large barrel-shaped structures together with components such as TEP1 and vault-associated RNAs. These complexes are implicated in intracellular transport processes, potentially mediating movement of macromolecules between the nucleus and cytoplasm. Immunofluorescence analysis using a Major vault protein antibody allows researchers to visualize these complexes in situ and assess how their distribution changes across different experimental conditions.
Functionally, MVP is strongly associated with multidrug resistance in cancer. Increased MVP expression has been reported in lung, breast, ovarian, and hematologic malignancies, where it contributes to reduced intracellular drug accumulation and altered apoptotic signaling. Immunofluorescence studies extend this understanding by revealing how MVP localization shifts within resistant cells, providing additional insight beyond expression levels alone.
Beyond drug resistance, MVP is involved in signaling pathways such as PI3K-AKT and has been linked to regulation of apoptosis, autophagy, and immune responses. Changes in intracellular distribution detected by immunofluorescence can therefore reflect broader alterations in cellular signaling and stress adaptation. Proper fixation and permeabilization are important for preserving MVP localization, as the protein is intracellular and associated with large ribonucleoprotein structures.
The MVP gene is located on chromosome 16p11.2 and encodes a protein composed of repeating structural domains that assemble into the characteristic vault particle. While MVP is primarily cytoplasmic, its dynamic localization and interaction with multiple signaling pathways highlight its functional complexity. Immunofluorescence using a Major vault protein antibody enables detailed visualization of these patterns at the single-cell level.
This MVP immunofluorescence antibody is suitable for detecting Major vault protein localization in cultured cells and tissue sections, supporting studies of intracellular transport, drug resistance, and spatial regulation of signaling pathways.
This MVP antibody is part of a broader collection of research tools designed to support studies in cancer biology, intracellular transport, and drug resistance mechanisms.
Optimal dilution of the Major Vault Protein Antibody for IF / MVP Immunofluorescence Antibody should be determined by the researcher.
Proteins precipitated from human breast cancer MCF-7 cells were used as the immunogen for the Major Vault Protein antibody.
Store the Major Vault Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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