MVP Antibody for FACS / Flow Cytometry Antibody [clone 8B12] (RQ5611)

IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

MVP Antibody Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human colon cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE mouse intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Western blot testing of human 1) A549, 2) U-2 OS, 3) HepG2, 4) ThP-1, 5) HeLa, 6) SW620, 7) rat RH35 and 8) mouse RAW264.7 lysate with MVP antibody. Observed molecular weight: 104~110 kDa.

MVP Antibody for FACS in Human A431 Cells. Flow cytometry analysis of Major Vault Protein (MVP / LRP) expression in human A431 cells using a mouse monoclonal antibody clone 8B12. Cells were fixed, permeabilized, and stained at 1 ug per million cells. The MVP antibody (blue) shows a clear rightward shift in fluorescence intensity compared to the isotype control (green) and unstained cells (red), indicating specific intracellular detection. The distinct population separation supports accurate gating and quantitative single-cell analysis, consistent with the known expression of Major vault protein in epithelial cancer cells and its role in drug resistance and cellular stress response pathways.

MVP Antibody for FACS in Human A549 Cells. Flow cytometry analysis of Major Vault Protein (MVP / LRP) expression in human A549 cells using a mouse monoclonal antibody (8B12). Cells were fixed, permeabilized, and stained at 1 ug per million cells. The MVP antibody (blue) demonstrates a clear rightward shift relative to the isotype control (green) and unstained cells (red), indicating specific intracellular detection. The separation of populations supports reliable gating and quantitative single-cell analysis, consistent with the known expression of Major vault protein in epithelial-derived cancer cells and its role in drug resistance and cellular stress response pathways.