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Home >> Antibodies >> CD13 Antibody (Lap1)

CD13 Antibody (Lap1) [clone B-F10] (V2555)

  Catalog No Formulation Size Price (USD)  
Image V2555-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2555-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2555SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name B-F10
Purity Protein G affinity chromatography
UniProt P15144
Localization Cell surface
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml for 30 min at RT
Limitations This CD13 antibody is available for research use only.
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Description

Recognizes an integral membrane glycoprotein of 150kDa, identified as CD13 (also known as aminopeptidase-N). The CD13 antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV). The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.

Application Notes

Optimal dilution of the CD13 antibody should be determined by the researcher.

Immunogen

KG-1 myeloid cells were used as the immunogen for the CD13 antibody.

Storage

Store the CD13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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