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UBE2A/UBE2B Antibody / Ubiquitin-conjugating enzyme E2

Catalog No : FY12134
Applications : WB, IHC, IF, FACS, ELISA
Reactivity : Human
Format : Lyophilized
IHC analysis of UBE2A/UBE2B using anti-UBE2A/UBE2B antibody. UBE2A/UBE2B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-UBE2A/UBE2B antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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