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banner Search results for SPECC1L

SPECC1L Antibody / Cytospin A

Catalog No : FY12109
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of HepG2 cells using anti-SPECC1L antibody. Overlay histogram showing HepG2 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPECC1L antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Cytospin A Antibody / SPECC1L

Catalog No : F54361
Applications : IF, FACS, IHC-P, WB
Reactivity : Human
Format : Purified
IHC testing of FFPE human brain tissue with SPECC1L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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