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Home >> Antibodies >> SPECC1L Antibody / Cytospin A

SPECC1L Antibody / Cytospin A (F54361)

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  Catalog No Formulation Size Price (USD)  
Image F54361-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54361-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human brain tissue with SPECC1L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human brain tissue with SPECC1L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-251 MG cells with SPECC1L antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human HL60 cell lysate with SPECC1L antibody. Predicted molecular weight ~125 kDa.
Flow cytometry testing of fixed and permeabilized human HL60 cells with SPECC1L antibody; Blue=isotype control, Green= SPECC1L antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q69YQ0
Localization Cytoplasmic
Applications Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This SPECC1L antibody is available for research use only.
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Description

Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the SPECC1L antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 705-733 from the human protein was used as the immunogen for the SPECC1L antibody.

Storage

Aliquot the SPECC1L antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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