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banner Search results for SEC61

SEC61 Antibody

Catalog No : R34412
Applications : WB, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Cow, Dog, Pig
Format : Antigen affinity purified
Western blot testing of human cerebellum lysate with SEC61 antibody at 0.3ug/ml. Predicted molecular weight: ~52kDa.
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SEC61G Antibody / Sec61 subunit gamma

Catalog No : FY12253
Applications : WB, IHC, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunohistochemical staining of SEC61G using anti-SEC61G antibody. SEC61G was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SEC61G antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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SEC61B Antibody / Sec61 subunit beta

Catalog No : FY12360
Applications : ELISA, FACS, WB
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of PC-3 cells using anti-SEC61B antibody. Overlay histogram showing PC-3 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC61B antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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