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RAPH1 Antibody / Ras-associated and pleckstrin homology domains-containing protein 1

Catalog No : FY12411
Applications : WB, IHC, ELISA
Reactivity : Human, Mouse
Format : Lyophilized
Immunohistochemical staining of RAPH1 using anti-RAPH1 antibody. RAPH1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAPH1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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