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banner Search results for Peroxin 1

PEX1 Antibody / Peroxin 1

Catalog No : FY12288
Applications : WB, FACS, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Flow Cytometry analysis of mouse Neuro-2a cells using anti-Peroxin 1 antibody. Overlay histogram showing Neuro-2a cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxin 1 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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PEX1 Antibody / Peroxisome biogenesis factor 1

Catalog No : FY12817
Applications : WB, IHC, IF, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Immunofluorescent staining of PEX1 using anti-PEX1 antibody (red). PEX1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-PEX1 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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PEX1 Antibody

Catalog No : R35193
Applications : WB, ELISA (peptide)
Reactivity : Human, Mouse, Rat
Pred. Reactivity :Cow, Dog
Format : Antigen affinity purified
Western blot testing of mouse liver lysate with Pex1 antibody at 0.3ug/ml. Predicted molecular weight: ~137kDa, observed here at ~150kDa.
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