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PTPRR Antibody / Protein tyrosine phosphatase receptor type R (clone 29P99)

Catalog No : FY12004
Applications : WB, IHC
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of PTPRR using anti-PTPRR antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPRR antibody at 1:500 overnight at 4oC, then washed with TBS-10%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for PTPRR at approximately 70 kDa. The expected band size for PTPRR is at 70 kDa.
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