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NEK5 Antibody / NimA-related protein kinase 5

Catalog No : FY12391
Applications : WB, IHC, ICC/IF, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of NEK5 using anti-NEK5 antibody (green) and anti-Beta Tubulin antibody (red). NEK5 was detected in an immunocytochemical section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-NEK5 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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