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NASP antibody / Nuclear autoantigenic sperm

Catalog No : FY12266
Applications : WB, IHC, ICC, IF, IP, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Liquid
Immunofluorescent staining of NASP using anti-NASP antibody (red). NASP was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-NASP antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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