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  • Tel: 858.663.9055
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banner Search results for Myosin

MYH2 Antibody / Myosin heavy chain 2 (clone 32M67)

Catalog No : FY12576
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Liquid
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MYL2 Antibody / Myosin regulatory light chain 2

Catalog No : FY13066
Applications : WB
Reactivity : Human, Monkey, Mouse, Rat
Format : Lyophilized
Western blot analysis of Myosin Light Chain 2/MLC-2V/MYL2 using anti-MYL2 antibody. Lane 1: human U20S whole cell lysates, Lane 2: monkey heart tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for Myosin Light Chain 2/MLC-2V/MYL2 at approximately 19 kDa. The expected molecular weight of Myosin Light Chain 2/MLC-2V/MYL2 is at 19 kDa.
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MYLK3 Antibody / Myosin light chain kinase 3

Catalog No : FY13284
Applications : WB, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of human HEL cells using anti-MYLK3 antibody. Overlay histogram showing HEL cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYLK3 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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MYL3 Antibody / Myosin light chain 3

Catalog No : FY13160
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of MYL3 using anti-MYL3 antibody. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human H9C2(2-1) whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL3 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. MYL3 antibody detects a band at ~24-25 kDa, often appearing as a doublet consistent with phosphorylation of the essential myosin light chain (predicted 22 kDa).
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MYL4 Antibody / Myosin light chain 4

Catalog No : FY12729
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of MYL4 using anti-MYL4 antibody. Lane 1: human K562 whole cell lysates, Lane 2: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected molecular weight of MYL4 is ~22 kDa.
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MYL5 Antibody / Myosin light chain 5

Catalog No : RQ8448
Applications : WB, IHC-P, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human U-2 OS cells with MYL5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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MYH6 Antibody / Myosin 6

Catalog No : RQ6780
Applications : WB, IHC-P, FACS
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
IHC staining of FFPE human heart tissue with Myosin 6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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MYL6 Antibody / Myosin light chain 6

Catalog No : FY13297
Applications : WB, FACS
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of human Hela cells using anti-MYL6 antibody. Overlay histogram showing Hela cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYL6 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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MYH6 Antibody / Myosin 6 (clone AOBH-13)

Catalog No : RQ5404
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Purified
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Western blot testing of mouse heart lysate with MYH6 antibody. Predicted molecular weight ~224 kDa, routinely observed at 200-250 kDa.
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MYH7 Antibody / Myosin 7 (clone MYH7/13050R)

Catalog No : V5768
Applications : IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human skeletal muscle tissue with recombinant Myosin 7 antibody (clone MYH7/13050R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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