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MED28 Antibody / Mediator complex subunit 28

Catalog No : FY12612
Applications : ELISA, FACS, IP, WB
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of THP-1 cells using anti-MED28 antibody. Overlay histogram showing THP-1 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED28 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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