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banner Search results for MASTL

MASTL Antibody / GWL

Catalog No : RQ7765
Applications : WB, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
Immunofluorescent staining of FFPE human U-2 OS cells with GWL antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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MASTL Antibody / Microtubule-associated serine/threonine-protein kinase-like

Catalog No : FY12096
Applications : WB, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of MASTL using anti-MASTL antibody. Lane 1: human U2OS whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASTL antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected band size for MASTL is at 96 kDa but it is commonly observed at 105-115 kDa due to phosphorylation.
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