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JAG1 Antibody / JAG1B / Zebrafish

Catalog No : RZ1400
Applications : WB, IHC, IF
Reactivity : Zebrafish
Format : Antigen affinity purified
Zebrafish JAG1 Antibody IF. Immunofluorescent staining of paraffin-embedded zebrafish embryo tissue using Zebrafish JAG1 Antibody / Jagged 1b Antibody demonstrates green fluorescence within developing embryonic structures, including regions associated with organogenesis and tissue patterning. The staining pattern is consistent with expression of JAG1B, a transmembrane Notch ligand that mediates cell-cell communication during vertebrate development. JAG1B plays important roles in cell fate determination, differentiation, tissue morphogenesis, and regulation of Notch signaling pathways. HIER was performed using EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 5 ug/ml overnight at 4C. DyLight-488-conjugated goat anti-rabbit IgG was used as the secondary antibody (green), and nuclei were counterstained with DAPI (blue). The image supports detection of JAG1B expression in developing zebrafish embryonic tissues by immunofluorescence.
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