Zebrafish JAG1 Antibody / Jagged 1b Antibody (RZ1400)

Zebrafish JAG1 Antibody IF. Immunofluorescent staining of paraffin-embedded zebrafish embryo tissue using Zebrafish JAG1 Antibody / Jagged 1b Antibody demonstrates green fluorescence within developing embryonic structures, including regions associated with organogenesis and tissue patterning. The staining pattern is consistent with expression of JAG1B, a transmembrane Notch ligand that mediates cell-cell communication during vertebrate development. JAG1B plays important roles in cell fate determination, differentiation, tissue morphogenesis, and regulation of Notch signaling pathways. HIER was performed using EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 5 ug/ml overnight at 4C. DyLight-488-conjugated goat anti-rabbit IgG was used as the secondary antibody (green), and nuclei were counterstained with DAPI (blue). The image supports detection of JAG1B expression in developing zebrafish embryonic tissues by immunofluorescence.

Zebrafish JAG1 Antibody Pancreas IHC. Immunohistochemistry staining of paraffin-embedded zebrafish pancreas tissue using Zebrafish JAG1 Antibody / Jagged 1b Antibody demonstrates strong HRP-DAB brown staining within a discrete cluster of pancreatic cells. The staining pattern is consistent with expression of JAG1B, a transmembrane Notch ligand that regulates cell-cell communication, cellular differentiation, and tissue patterning during vertebrate development. JAG1B-mediated Notch signaling plays important roles in pancreatic development, maintenance of progenitor cell populations, and regulation of cell fate decisions within developing tissues. HIER was performed using EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/ml overnight at 4C prior to detection with peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen.

Zebrafish JAG1 Antibody WB. Western blot analysis of zebrafish head tissue lysate using Zebrafish JAG1 Antibody / Jagged 1b Antibody demonstrates a prominent immunoreactive band at approximately 150-155 kDa, consistent with expression of JAG1B. Jagged 1b is a transmembrane Notch ligand that mediates cell-cell communication required for cell fate determination, tissue patterning, differentiation, and organ development during vertebrate embryogenesis. The observed molecular weight exceeds the predicted size of approximately 133 kDa, which is consistent with the extensive glycosylation known to occur within Jagged family proteins. Additional lower molecular weight bands are present and may represent processed forms, degradation products, or nonspecific reactivity. Thirty micrograms of protein were loaded under reducing conditions. Predicted molecular weight: approximately 133 kDa.