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banner Search results for GUCY2D

GUCY2D Antibody / Guanylate cyclase 2D

Catalog No : FY12112
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of GUCY2D using anti-GUCY2D antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human 293T whole cell lysates, Lane 2: human whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GUCY2D antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for GUCY2D at approximately 120 kDa. The expected band size for GUCY2D is at 120 kDa.
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Retinal guanylyl cyclase 1 Antibody / GUCY2D

Catalog No : F54602
Applications : WB, IHC-P, FACS
Reactivity : Human, Rat
Format : Purified
IHC testing of FFPE human thyroid tissue with Retinal guanylyl cyclase 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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