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banner Search results for FOXM1

FOXM1 Antibody / Forkhead box protein M1

Catalog No : FY12211
Applications : WB, ICC, IF, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of FOXM1 using anti-FOXM1 antibody (green) and anti-Beta Tubulin antibody (red). FOXM1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-FOXM1 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight 550 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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FOXM1 Antibody

Catalog No : R31607
Applications : WB
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of FOXM1 antibody and Lane 1: HeLa; 2: COLO320; 3: SW620; 4: SKOV; 5: MCD-7. Predicted/observed size ~84KD
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FOXM1 Antibody / Forkhead box protein M1

Catalog No : FY12831
Applications : WB, IHC, ELISA, IP
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Western blot analysis of FOXM1 using anti-FOXM1 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human DLD1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXM1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Major bands between ~85-105 kDa correspond to FOXM1 isoforms and phosphorylation states. The multiple closely spaced bands represent differentially phosphorylated forms of FOXM1, consistent with its activation during the G2/M phase of the cell cycle.
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