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  • Email: info@nsjbio.com
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ESPN Antibody / Espin

Catalog No : FY12640
Applications : WB, ELISA
Reactivity : Mouse, Rat
Format : Lyophilized
Western blot analysis of ESPN using anti-ESPN antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESPN antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Western blot probed with anti-ESPN shows a major band at ~100 kDa in rat testis and ~110 kDa in mouse testis, higher than the predicted ~92 kDa, consistent with species-specific espin isoforms and phosphorylated forms expressed during spermatogenesis.
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Espin Antibody / ESPN

Catalog No : F54893
Applications : FACS, IHC-P, WB
Reactivity : Human
Format : Purified
Western blot testing of human SK-BR-3 cell lysate with Espin antibody. Predicted molecular weight ~92 kDa; isoforms can be observed at ~110 kDa and ~30 kDa.
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