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CIAO3 Antibody / Cytosolic iron-sulfur assembly component 3

Catalog No : FY12486
Applications : WB, FACS, ELISA
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of MCF-7 cells using anti-CIAO3 antibody. Overlay histogram showing MCF-7 cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CIAO3 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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