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DBT Antibody / Dihydrolipoamide branched chain transacylase E2 / BCKDH E2

Catalog No : FY13381
Applications : WB, ICC/IF, IP, FACS, ELISA
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of BCKDH E2/DBT using anti-BCKDH E2 antibody (green) and anti-Beta Tubulin antibody (red). BCKDH E2/DBT was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-BCKDH E2 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight 550 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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