BCKDH E2 Antibody / DBT Antibody (FY13381)

BCKDH E2 Antibody detection in U2OS cells by IF. Immunofluorescence staining of human U2OS cells using the BCKDH E2 Antibody (green) with Beta Tubulin antibody (red). BCKDH E2 shows a granular cytoplasmic distribution surrounding the blue DAPI-stained nuclei, consistent with the mitochondrial localization of DBT. Beta Tubulin highlights the cytoskeletal network and provides clear spatial contrast with the BCKDH E2 signal. Enzyme-mediated antigen retrieval was performed for 15 minutes before overnight primary antibody incubation at 4oC. The observed staining demonstrates intracellular detection of dihydrolipoamide branched-chain transacylase E2 with this DBT Antibody.

BCKDH E2 Antibody detection across human, rat and mouse samples by WB. Western blot testing with the BCKDH E2 Antibody. Lane 1: human SiHa; lane 2: human cell lysate; lane 3: human HaCaT; lane 4: human Jurkat; lane 5: rat stomach; lane 6: rat kidney; lane 7: mouse stomach; lane 8: mouse kidney. A strong predominant band is detected just below 50 kDa across the samples. This migration is slightly below the predicted 53.5 kDa molecular weight but is consistent with the approximately 52 kDa processed mitochondrial form of DBT. The clean cross-species signal demonstrates recognition with this DBT Antibody.

BCKDH E2 Antibody immunoprecipitation of DBT by IP. Immunoprecipitation of BCKDH E2 from whole-cell lysate followed by western blot detection with the BCKDH E2 Antibody. Lane 1: 30 ug whole-cell lysate input; lane 2: rabbit control IgG with whole-cell lysate; lane 3: 2 ug BCKDH E2 Antibody with 500 ug whole-cell lysate. A distinct band just below 50 kDa is present in the input and BCKDH E2 immunoprecipitate while the control IgG lane is negative. The result demonstrates selective enrichment and detection of dihydrolipoamide branched-chain transacylase E2 with this DBT Antibody.

BCKDH E2 Antibody detection in SiHa cells by FACS. Flow cytometry analysis of fixed and permeabilized human SiHa cells using the BCKDH E2 Antibody at 1 ug per million cells. The blue antibody-treated population shows a clear rightward fluorescence shift relative to the green rabbit IgG isotype control and red unstained control. Cells were blocked with normal goat serum before primary antibody incubation and detected with a DyLight 488-conjugated secondary antibody. The distinct fluorescence shift demonstrates intracellular detection of dihydrolipoamide branched-chain transacylase E2 with this DBT Antibody.

BCKDH E2 Antibody staining of human kidney by IHC. Immunohistochemistry of FFPE human kidney sections using the BCKDH E2 Antibody at 2 ug/ml. Strong granular brown cytoplasmic staining is prominent within renal tubular epithelial cells, while glomerular structures show comparatively little staining. Nuclei are counterstained blue and remain largely negative. Heat-mediated antigen retrieval was performed in pH 8 EDTA before antibody incubation. The predominantly cytoplasmic pattern is consistent with the mitochondrial localization of DBT and demonstrates tissue detection of dihydrolipoamide branched-chain transacylase E2 with this DBT Antibody.

BCKDH E2 Antibody staining of human liver cancer by IHC. Immunohistochemistry of FFPE human liver cancer sections using the BCKDH E2 Antibody at 2 ug/ml. Moderate-to-strong brown granular staining is distributed throughout the cytoplasm of many tumor cells, while nuclei are counterstained blue and remain predominantly negative. Staining intensity varies among individual cells but maintains a primarily cytoplasmic distribution consistent with mitochondrial DBT localization. Heat-mediated antigen retrieval was performed in pH 8 EDTA before antibody incubation. The observed staining demonstrates tissue detection of dihydrolipoamide branched-chain transacylase E2 with this DBT Antibody.