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banner Search results for ATP4B

ATP4B Antibody / Potassium-transporting ATPase subunit beta

Catalog No : FY12265
Applications : ELISA, FACS, IF, IHC, WB
Reactivity : Human, Mouse, Rat
Format : Lyophilized
Immunofluorescent staining of ATP4B using anti-ATP4B antibody (red). ATP4B was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-ATP4B antibody overnight at 4oC. DyLight 594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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ATP4B Antibody

Catalog No : R32734
Applications : WB
Reactivity : Human, Mouse, Rat
Format : Antigen affinity purified
Western blot testing of 1) rat stomach, 2) mouse stomach and 3) human SGC-7901 (human gastric cancer) lysate with ATP4B antibody at 0.5ug/ml. Predicted molecular weight ~34 kDa (core peptide), ~52 kDa (beta subunit precursor), 60-80 kDa (glycosylated form).
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