NSJ Bioreagents

Aquaporin 4 (AQP4) is a crucial protein found in the central nervous system that plays a vital role in water transport and balance. Understanding the expression and localization of AQP4 is essential for studying conditions such as brain edema, multiple sclerosis, and neuromyelitis optica. One of the primary tools used by researchers to study Aquaporin 4 is the Aquaporin 4 antibody. This antibody specifically targets and detects the Aquaporin 4 protein in various biological samples.

Western blotting is a common technique used to analyze the expression levels of Aquaporin 4 in cell lysates or tissue homogenates. By using the Aquaporin 4 antibody in western blotting, researchers can determine the size and quantity of the AQP4 protein present.

Immunohistochemistry is another valuable method for visualizing the localization of Aquaporin 4 within tissues. By applying the Aquaporin 4 antibody to tissue sections, researchers can identify the cellular distribution and subcellular localization of Aquaporin 4.

Immunofluorescence is a similar technique that allows for high-resolution imaging of Aquaporin 4 in cells or tissues, making it ideal for studying the dynamic changes in Aquaporin 4 expression and distribution.

Flow cytometry is a powerful tool for analyzing Aquaporin 4 expression at the single-cell level. By using the Aquaporin 4 antibody in flow cytometry, researchers can quantify the amount of Aquaporin 4 present in different cell populations and investigate how Aquaporin 4 expression may change in response to various stimuli.

The use of the Aquaporin 4 antibody in techniques such as western blotting, immunohistochemistry, immunofluorescence, and flow cytometry can provide valuable insights into the expression and function of Aquaporin 4. By utilizing these detection methods, researchers can further expand our understanding of the role of Aquaporin 4 in health and disease.