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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2561 to 2570
    MYH7 Antibody / Myosin 7 (clone MYH7/9969R)
    Catalog No : V5955
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of Beta-Myosin Heavy Chain / MYH7 antibody (clone MYH7/9969R). Skeletal muscle tissue lysates from human, mouse, rat, hamster, guinea pig, and rabbit were resolved by SDS-PAGE and probed with Beta-Myosin Heavy Chain / MYH7 antibody (clone MYH7/9969R), demonstrating a prominent immunoreactive band at approximately 220 kDa consistent with the predicted molecular weight of MYH7. The observed band corresponds to beta-myosin heavy chain expressed in slow-twitch skeletal muscle fibers across species.
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    CEACAM6 Antibody / Carcinoembryonic antigen-related cell adhesion molecule 6 (clone CEACAM6/13371R)
    Catalog No : V5956
    Applications : FACS, IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of CEACAM6 antibody (clone CEACAM6/13371R). Whole cell lysates from human HT-29 and AsPC-1 cells were resolved by SDS-PAGE and probed with CEACAM6 antibody (clone CEACAM6/13371R), demonstrating a prominent immunoreactive band at approximately 90-100 kDa consistent with the predicted molecular weight of glycosylated CEACAM6. The observed band pattern aligns with known expression of CEACAM6 in colorectal and pancreatic carcinoma cell lines.
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    CEACAM6 Antibody / CD66c (clone CEACAM6/13372R)
    Catalog No : V5957
    Applications : FACS, IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of CD66c / CEACAM6 antibody in human HT-29 clel lysate. A distinct band is observed at approximately 90-100 kDa in human HT-29 cell lysate, consistent with the predicted molecular weight of CEACAM6 and its known glycosylated form. Clone CEACAM6/13372R detects CD66c protein under reducing conditions, demonstrating specific recognition of CEACAM6 in human colorectal adenocarcinoma cells.
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    CEACAM6 Antibody / Nonspecific cross-reacting antigen 90 (clone CEACAM6/10079R)
    Catalog No : V5958
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of Nonspecific Cross-Reacting Antigen 90 / CEACAM6 antibody. Human HT-29 and AsPC-1 whole cell lysates show a prominent band at approximately 90-100 kDa, consistent with the predicted molecular weight of CEACAM6 and its heavily glycosylated mature form. Clone CEACAM6/10079R detects CEACAM6 protein under reducing conditions, demonstrating specific recognition in human colorectal and pancreatic adenocarcinoma cell lines.
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    NME2 Antibody / Nucleoside diphosphate kinase B (clone NME2/12953R)
    Catalog No : V5959
    Applications : WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western Blot Analysis of nm23-H2 / NME2 antibody (clone NME2/12953R). Human HeLa and human MCF7 whole cell lysates show a distinct immunoreactive band at approximately 17 kDa, consistent with the predicted molecular weight of NME2. The band appears as a single, sharp species in both cell lines with no significant non-specific background under reducing conditions, supporting specific detection of nm23-H2 protein.
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    PCNA Antibody / Proliferating cell nuclear antigen (clone PCNA/13725R)
    Catalog No : V5964
    Applications : WB
    Reactivity : Human, Mouse, Rat, Hamster, Rabbit, Guinea pig
    Format : Purified
    Recrabbitmono
    Western blot analysis of PCNA Proliferation Marker antibody in multi-species skin lysates. Western blot was performed using PCNA Proliferation Marker antibody (clone PCNA/13725R) on human skin, mouse skin, rat skin, hamster skin, guinea pig skin, and rabbit skin tissue lysates. A clear immunoreactive band is detected at approximately 29 kDa in all species tested, consistent with the predicted molecular weight of Proliferating Cell Nuclear Antigen. The conserved band size across species reflects the high sequence conservation of PCNA among mammals. The detected signal corresponds to the predicted molecular weight under reducing conditions. PCNA is a nuclear replication factor expressed in proliferating cells within skin tissue, and the consistent band pattern supports specific recognition of endogenous PCNA across multiple mammalian species.
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    Perforin Antibody / PRF1 (clone PRF1/9681R)
    Catalog No : V5975
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of Cytotoxic Lymphocyte Perforin Antibody in HDLM-2 cell lysate. HDLM-2 lysate shows a prominent band at approximately 70 kDa, consistent with the predicted molecular weight of Perforin-1. The signal appears as a strong, specific band under reducing SDS-PAGE conditions, with minor higher molecular weight signal that may represent protein aggregation or incompletely reduced species. The observed band aligns with endogenous PRF1 expression, which is expected in activated or cytotoxic lymphoid cell populations.
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    PTPRC Antibody / CD45 (clone MSVA-045R)
    Catalog No : V5980
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of Leukocyte Common Antigen/CD45RA antibody in human tissue microarrays. Formalin-fixed, paraffin-embedded human normal and cancer tissue microarrays were stained with Leukocyte Common Antigen/CD45RA antibody (clone MSVA-045R). Robust membranous HRP-DAB brown staining is observed in lymphoid-rich tissues including tonsil, lymph node, spleen, and thymus, consistent with expression of CD45RA on subsets of hematopoietic cells, while most non-hematopoietic epithelial tissues show minimal to absent staining. In the cancer tissue gallery, strong staining is detected in multiple lymphoma subtypes, whereas the majority of non-hematologic malignancies demonstrate limited reactivity. Negative control sections processed with PBS instead of primary antibody show no specific staining. Heat-induced epitope retrieval was performed by boiling sections in 10mM Tris with 1mM EDTA, pH 9.0, followed by cooling prior to immunostaining.
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    PVALB Antibody / Parvalbumin (clone PVALB/12794R)
    Catalog No : V5983
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of Calcium-Binding Protein PV/PVALB antibody (clone PVALB/12794R) in brain tissue lysates from multiple species. Brain lysates from human, mouse, and rat show clear immunoreactive bands at approximately 12 kDa, consistent with the predicted molecular weight of Parvalbumin based on its amino acid sequence. No specific band is observed in hamster brain, guinea pig brain, or rabbit brain under the conditions tested. The detected bands migrate in the expected low molecular weight range for this small cytosolic EF-hand calcium-binding protein, supporting species-reactive detection of Parvalbumin in human, mouse, and rat brain tissue.
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    PVALB Antibody / Parvalbumin (clone PVALB/12610R)
    Catalog No : V5984
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of Fast-Spiking Interneuron Marker/Parvalbumin antibody (clone PVALB/12610R) in human kidney tissue. FFPE human kidney shows cytoplasmic HRP-DAB brown staining within renal tubular epithelial cells, consistent with Parvalbumin expression. Staining is localized predominantly to the cytoplasm of tubular structures, while adjacent glomerular elements and surrounding interstitial cells demonstrate minimal specific signal. The inset image represents a secondary antibody negative control in which PBS was used in place of the primary antibody and shows absence of specific staining. Heat induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, at 95oC for 45 minutes followed by cooling at room temperature for 20 minutes prior to immunostaining.
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