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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2561 to 2570
    MCM5 Antibody / Replication licensing factor MCM5 (clone MCM5/13320R)
    Catalog No : V5945
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    MCM5 Antibody Knockdown Validation HeLa. Knockdown validation of MCM5 expression in human HeLa cells using MCM5 antibody clone MCM5/13320R. Lane 1: wild-type cells, Lane 2: MCM5 shRNA knockdown cells. A marked reduction in band intensity is observed in knockdown samples compared to wild-type controls, confirming specific detection of endogenous MCM5. Beta-Tubulin is used as a loading control.
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    CD99 Antibody / CD99 molecule (clone MIC2/9939R)
    Catalog No : V5946
    Applications : IHC, WB
    Reactivity : Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human Ewing sarcoma tissue with recombinant MIC2/CD99 antibody (clone MIC2/9939R). Tumor cells show strong diffuse membranous staining. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 45 min at 95oC followed by cooling at RT for 20 minutes.
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    CD10 Antibody / Neprilysin (clone MME/12957R)
    Catalog No : V5948
    Applications : IHC, WB
    Reactivity : Human, Mouse, Rat
    Format : Purified
    Recrabbitmono
    IHC staining of FFPE human kidney tissue with MME/CD10 antibody (clone MME/12957R). Membranous and luminal staining is observed in renal proximal tubular epithelial cells, consistent with known CD10 expression, while surrounding interstitial cells show minimal staining. Inset: PBS used instead of primary antibody as a negative control. Heat-induced epitope retrieval was performed by boiling sections in pH 9 10 mM Tris with 1 mM EDTA for 45 min at 95oC followed by cooling at RT for 20 min.
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    MUC1 Antibody for IHC / Mucin 1 (clone MSVA-672R)
    Catalog No : V5950
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    MUC1 Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Mucin 1 MUC1, also known as Epithelial membrane antigen, in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant rabbit monoclonal MUC1 antibody clone MSVA-672R. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates prominent membranous and cytoplasmic localization in glandular and ductal epithelial tissues, consistent with luminal surface expression, while non-epithelial tissues including stromal and mesenchymal compartments show minimal to absent staining. Within tumor tissue microarrays, strong and often diffuse staining is observed in epithelial-derived malignancies, supporting identification of carcinoma cells and altered epithelial polarity. Evaluation across large TMA panels enables direct comparison of MUC1 expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported MUC1 expression profiles in publicly available datasets including the Human Protein Atlas.
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    MUC4 Antibody / Mucin 4 (clone MUC4/8660R)
    Catalog No : V5952
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Immunohistochemistry analysis of recombinant Sialomucin Complex Subunit 4 / MUC4 antibody (clone MUC4/8660R) in human colon tissue. Formalin-fixed, paraffin-embedded human colon section shows membranous and apical cytoplasmic brown chromogenic staining in epithelial cells lining glandular structures, consistent with MUC4 expression, while surrounding stromal cells exhibit minimal staining and nuclei appear blue. The inset shows PBS used in place of primary antibody as a negative control with no specific staining observed. Heat-induced epitope retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.
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    MUC6 Antibody for IHC / Mucin 6 (clone MSVA-806R)
    Catalog No : V5954
    Applications : IHC
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    MUC6 Antibody for IHC Tissue Microarray (TMA). Immunohistochemistry analysis of Mucin 6 MUC6 in formalin-fixed paraffin-embedded human normal and cancer tissue microarrays using recombinant rabbit monoclonal MUC6 antibody clone MSVA-806R. Tissue microarray (TMA) staining with HRP-DAB brown chromogen demonstrates distinct cytoplasmic localization in pyloric gland epithelium and Brunner glands, consistent with gastric-type mucin expression, while non-glandular and non-epithelial compartments including stromal and mesenchymal tissues show minimal to absent staining. Additional cytoplasmic staining is observed in select glandular epithelial tissues. Within tumor tissue microarrays, positive staining is detected in a subset of glandular and mucinous carcinomas, supporting identification of tumors with gastric or pyloric differentiation, while many non-mucinous malignancies remain largely negative. Evaluation across large TMA panels enables direct comparison of MUC6 expression across diverse tissue types under standardized conditions. The observed staining patterns align with reported MUC6 expression profiles in publicly available datasets including the Human Protein Atlas.
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    MYH7 Antibody / Myosin 7 (clone MYH7/9969R)
    Catalog No : V5955
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of Beta-Myosin Heavy Chain / MYH7 antibody (clone MYH7/9969R). Skeletal muscle tissue lysates from human, mouse, rat, hamster, guinea pig, and rabbit were resolved by SDS-PAGE and probed with Beta-Myosin Heavy Chain / MYH7 antibody (clone MYH7/9969R), demonstrating a prominent immunoreactive band at approximately 220 kDa consistent with the predicted molecular weight of MYH7. The observed band corresponds to beta-myosin heavy chain expressed in slow-twitch skeletal muscle fibers across species.
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    CEACAM6 Antibody / Carcinoembryonic antigen-related cell adhesion molecule 6 (clone CEACAM6/13371R)
    Catalog No : V5956
    Applications : FACS, IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot analysis of CEACAM6 antibody (clone CEACAM6/13371R). Whole cell lysates from human HT-29 and AsPC-1 cells were resolved by SDS-PAGE and probed with CEACAM6 antibody (clone CEACAM6/13371R), demonstrating a prominent immunoreactive band at approximately 90-100 kDa consistent with the predicted molecular weight of glycosylated CEACAM6. The observed band pattern aligns with known expression of CEACAM6 in colorectal and pancreatic carcinoma cell lines.
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    CEACAM6 Antibody / CD66c (clone CEACAM6/13372R)
    Catalog No : V5957
    Applications : FACS, IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of CD66c / CEACAM6 antibody in human HT-29 clel lysate. A distinct band is observed at approximately 90-100 kDa in human HT-29 cell lysate, consistent with the predicted molecular weight of CEACAM6 and its known glycosylated form. Clone CEACAM6/13372R detects CD66c protein under reducing conditions, demonstrating specific recognition of CEACAM6 in human colorectal adenocarcinoma cells.
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    CEACAM6 Antibody / Nonspecific cross-reacting antigen 90 (clone CEACAM6/10079R)
    Catalog No : V5958
    Applications : IHC, WB
    Reactivity : Human
    Format : Purified
    Recrabbitmono
    Western blot testing of Nonspecific Cross-Reacting Antigen 90 / CEACAM6 antibody. Human HT-29 and AsPC-1 whole cell lysates show a prominent band at approximately 90-100 kDa, consistent with the predicted molecular weight of CEACAM6 and its heavily glycosylated mature form. Clone CEACAM6/10079R detects CEACAM6 protein under reducing conditions, demonstrating specific recognition in human colorectal and pancreatic adenocarcinoma cell lines.
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