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Home >> Antibodies >> MCM complex component 5 Antibody / MCM5

MCM complex component 5 Antibody / MCM5 [clone MCM5/13320R] (V5945)

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  Catalog No Formulation Size Price (USD)  
Image V5945-100UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 559
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V5945-20UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 259
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V5945SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Immunohistochemistry of recombinant MCM complex component 5 antibody in formalin-fixed, paraffin-embedded human tonsil. Strong nuclear brown staining is observed in proliferating lymphoid cells within germinal centers, consistent with MCM5 expression in actively cycling cells, while surrounding non-proliferating cells show weaker or absent staining. Inset: PBS used in place of primary antibody as a negative control shows no specific staining. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature.
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Immunohistochemistry of recombinant MCM complex component 5 antibody in formalin-fixed, paraffin-embedded human tonsil. Strong nuclear brown staining is observed in proliferating lymphoid cells within germinal centers, consistent with MCM5 expression in actively cycling cells, while surrounding non-proliferating cells show weaker or absent staining. Inset: PBS used in place of primary antibody as a negative control shows no specific staining. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature.
Western Blot analysis of human Ramos and Raji cell lysates using recombinant MCM complex component 5/MCM5 antibody (clone MCM5/13320R). Expected molecular weight: 80~90 kDa.
SDS-PAGE Analysis of purified recombinant MCM complex component 5/MCM5 antibody (clone MCM5/13320R). Confirmation of Purity and Integrity of Antibody.
Western Blot analysis of human A431 cell lysates using recombinant MCM complex component 5/MCM5 antibody (clone MCM5/13320R). Expected molecular weight: 80~90 kDa.
Western blot analysis of recombinant MCM complex component 5/MCM5 antibody in human HeLa cells. Lysates from wild-type (WT) and MCM5 shRNA knockdown (KD) cells were loaded at 30ug total protein per lane. A single band is detected at the predicted molecular weight of MCM5 in WT cells, with reduced intensity in shRNA knockdown cells, supporting target specificity. Beta-Tubulin is shown as a loading control.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MCM5/13320R
Purity Protein A affinity
UniProt P33992
Localization Chromosome, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Knockdown :
Western Blot : 2-4ug/ml
Limitations This MCM complex component 5/MCM5 antibody is available for research use only.
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Description

MCM complex component 5 Antibody recognizes MCM5, also known as Minichromosome maintenance complex component 5, a core subunit of the MCM2-7 helicase complex that drives DNA replication initiation and elongation. MCM5 antibody targets a nuclear protein essential for replication licensing, ensuring that genomic DNA is duplicated once per cell cycle. The MCM5 gene encodes a highly conserved ATP-dependent helicase component that participates in origin recognition, pre-replication complex assembly, and replication fork progression.

MCM5 is expressed in proliferating cells and localizes predominantly to the nucleus during G1, S, and G2 phases, where it forms a hexameric ring with MCM2, MCM3, MCM4, MCM6, and MCM7. This complex functions as the replicative helicase that unwinds double-stranded DNA ahead of the replication fork. Because MCM5 is present in cells licensed for replication but absent in quiescent or terminally differentiated cells, MCM complex component 5 Antibody is widely used as a sensitive marker of cell proliferation in both research and diagnostic pathology settings.

Compared with other proliferation markers such as Ki-67, MCM5 may identify a broader population of cells that are competent to enter S phase, including early G1-phase cells. This characteristic makes MCM5 antibody particularly valuable in studies of cell cycle regulation, tumor growth kinetics, and tissue regeneration. Increased nuclear expression of MCM5 has been reported in a wide range of malignancies, including colorectal, breast, lung, and urothelial carcinomas, where it often correlates with high proliferative index and aggressive tumor behavior.

At the molecular level, MCM5 activity is regulated through phosphorylation by cyclin-dependent kinases and DDK complexes, which coordinate helicase activation at replication origins. Dysregulation of MCM5 expression or replication licensing control can contribute to genomic instability, a hallmark of cancer. MCM complex component 5 Antibody provides a reliable tool for evaluating DNA replication competence, cell cycle progression, and proliferative status in normal tissues and neoplastic lesions. Clone MCM5/13320R is designed to detect nuclear MCM5 expression in research applications, supporting studies in oncology, cell biology, and replication stress pathways.

By targeting a fundamental component of the DNA replication machinery, MCM5 antibody enables precise assessment of proliferative activity and replication licensing dynamics across diverse biological systems.

Application Notes

1. Optimal dilution of the MCM complex component 5/MCM5 antibody should be determined by the researcher.

2. This MCM complex component 5/MCM5 antibody is recombinantly produced by expression in CHO cells.

Immunogen

A recombinant fragment (around amino acids 500-734) of human MCM5 protein (exact sequence is proprietary) was used as the immunogen for the MCM complex component 5/MCM5 antibody.

Storage

MCM5/Replication licensing factor MCM5 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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