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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2381 to 2390
    NFE2 Antibody / Nuclear factor erythroid 2 (clone 31N33)
    Catalog No : FY12867
    Applications : WB, IHC, ICC, IF, IP
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot testing using the NFE2 antibody at 1:2000 dilution for 1 hour at room temperature. Predicted molecular weight ~41 kDa, observed at 41-45 kDa.
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    MS4A14 Antibody / Membrane spanning 4 domains subfamily A member 14 (clone 29M80)
    Catalog No : FY12869
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of MS4A14 expression in human MCF7 cell lysate using MS4A14 antibody. Predicted molecular weight ~77 kDa.
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    TMF1 Antibody / TATA element modulatory factor (clone 30T88)
    Catalog No : FY12871
    Applications : WB, ICC, IF
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of TMF expression in human HeLa cell lysate using TMF1 antibody. TMF1 western blot shows a major band at ~160 kDa. Although the calculated mass is ~123 kDa, TMF1 is a coiled-coil Golgi scaffold that commonly migrates above its predicted size, consistent with the observed band.
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    TNFAIP8 Antibody / Tumor necrosis factor alpha induced protein 8 (clone 30T22)
    Catalog No : FY12872
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of TNFAIP8 expression in human A549 cell lysate using TNFAIP8 antibody. TNFAIP8 western blot shows a predominant band at ~19 kDa. Although the predicted mass is ~23 kDa, TNFAIP8 commonly migrates at ~18-21 kDa due to electrophoretic mobility characteristics of small acidic proteins and possible isoform/alternative-start usage.
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    IFT88 Antibody / Intraflagellar transport protein 88 (clone 29I92)
    Catalog No : FY12875
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of IFT88 expression in human HepG2 cell lysate using IFT88 antibody. Predicted molecular weight ~94 kDa.
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    RNF7 Antibody / Ring finger protein 7 (clone 30R91)
    Catalog No : FY12880
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of RNF7 expression in human HepG2 cell lysate. Predicted molecular weight ~13 kDa.
    img
    RAB8A Antibody / Ras related protein Rab 8A (clone 29R94)
    Catalog No : FY12883
    Applications : WB, IHC, ICC, IF, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of RAB8A expression in human HeLa cell lysate using RAB8A antibody. Predicted molecular weight ~24 kDa.
    img
    GNAQ Antibody / Guanine nucleotide binding protein Gq subunit alpha (clone 30G74)
    Catalog No : FY12887
    Applications : WB, IHC, ICC/IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of GNAQ using anti-GNAQ antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAQ antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The expected molecular weight of GNAQ is ~42 kDa.
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    NTHL1 Antibody / Endonuclease III like protein 1 (clone 30N17)
    Catalog No : FY12897
    Applications : WB, IHC, ICC, IF
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of NTH1 expression in human 293 cell lysate using NTHL1 antibody. Predicted molecular weight ~34 kDa.
    img
    CBR1 Antibody / Carbonyl reductase 1 (clone 30C98)
    Catalog No : FY12902
    Applications : WB, IHC, ICC, IF
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of CBR1 using anti-CBR1 antibody. CBR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CBR1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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