Antibodies for IHC, Western Blot, IF and Flow Cytometry

Antibody Applications in Protein Detection and Analysis

Primary antibodies for protein detection are widely used in research applications including immunohistochemistry (IHC), western blot, immunofluorescence (IF), and flow cytometry. These antibody-based techniques enable analysis of protein expression, localization, and function across tissues, cells, and biochemical samples, supporting studies in immunology, cancer biology, neuroscience, and metabolism.

Antibodies for IHC, western blot antibodies, immunofluorescence antibodies, and flow cytometry antibodies each provide distinct advantages depending on the experimental approach. Immunohistochemistry antibodies are used for tissue staining and biomarker detection in formalin-fixed samples, while western blot antibodies allow detection of protein size and expression levels in lysates. Immunofluorescence antibodies enable visualization of subcellular localization using fluorescent imaging, and flow cytometry antibodies support quantitative analysis of protein expression across cell populations.

Immunohistochemistry Antibodies

Immunohistochemistry antibodies are used for detecting protein expression in formalin-fixed, paraffin-embedded tissues, enabling visualization of cellular localization and tissue architecture. IHC antibodies are widely used in cancer research, pathology, and biomarker analysis, where spatial staining patterns provide critical insight into protein distribution and disease-associated expression.

Western Blot Antibodies

Western blot antibodies are used to detect specific proteins in cell and tissue lysates, providing information on molecular weight, protein expression levels, and post-translational modifications. Western blotting is a core method for validating protein identity and analyzing expression changes under different experimental conditions.

Immunofluorescence Antibodies

Immunofluorescence antibodies enable detection of proteins within cells using fluorescent labeling, allowing detailed visualization of subcellular localization and protein distribution. IF antibodies are widely used for imaging-based studies, co-localization analysis, and investigation of cellular structure and function.

Flow Cytometry Antibodies

Flow cytometry antibodies are used to analyze protein expression on the surface or within cells in suspension, enabling quantitative measurement across large cell populations. Flow cytometry is widely used in immunology and cell biology for identifying specific cell subsets and evaluating functional markers.

This antibody applications page is part of a broader antibody panel offered by NSJ Bioreagents.

A selection of antibody products is shown below to support a range of research applications.