- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
GAPDH Antibody [clone BG-7] (RQ5051)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5051 | Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA | 100 ul | 439 |
| Availability | 1-2 weeks |
| Species Reactivity | Human, Mouse, Rat |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | BG-7 |
| Purity | Affinity purified |
| UniProt | P04406 |
| Localization | Nuclear, cytoplasmic |
| Applications | Western Blot : 1:3000-1:10000 Immunohistochemistry (FFPE) : 1:100-1:250 Immunofluorescence : 1:100-1:250 |
| Limitations | This GAPDH antibody is available for research use only. |
|
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GAPDH antibody is a widely used reagent for research in cell biology, protein expression studies, and molecular assays. The encoded protein, glyceraldehyde 3 phosphate dehydrogenase (GAPDH), is a glycolytic enzyme that catalyzes the conversion of glyceraldehyde 3 phosphate to 1,3 bisphosphoglycerate. Because of its ubiquitous expression and stable abundance, GAPDH is frequently used as a housekeeping protein and loading control in western blotting and quantitative PCR studies.
GAPDH is expressed in virtually all tissues, reflecting its essential role in energy metabolism. Its steady expression across experimental conditions makes it an ideal reference protein for normalization. Detection with GAPDH antibody ensures accurate interpretation of protein loading and experimental outcomes. For this reason, GAPDH is one of the most cited internal controls in biomedical research.
Beyond glycolysis, GAPDH participates in multiple cellular processes. It is involved in DNA repair, nuclear transport, membrane fusion, and apoptosis. GAPDH translocation to the nucleus has been observed during oxidative stress, where it influences gene expression and cell survival. These non glycolytic functions highlight GAPDH as more than just a metabolic enzyme.
At the structural level, GAPDH is a tetrameric protein with conserved catalytic cysteine residues critical for enzymatic activity. Its high degree of evolutionary conservation across species makes GAPDH antibody applicable in a wide range of models, from bacteria to mammals. This versatility further reinforces its value as a reference protein in experimental biology.
The GAPDH antibody is widely applied in western blotting, immunohistochemistry, immunofluorescence, and ELISA. These applications support detection across tissues, cell lines, and experimental systems. For researchers seeking reliable normalization controls or exploring glycolysis and multifunctional protein roles, the GAPDH antibody is indispensable. NSJ Bioreagents supplies validated antibodies that ensure reproducibility and accuracy in molecular studies.
Optimal dilution of the GAPDH antibody should be determined by the researcher.
A synthetic peptide specific to human GAPDH was used as the immunogen for the GAPDH antibody.
Store the GAPDH antibody at -20oC.
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