TLE1 Antibody / Transcriptional Corepressor [clone TLE1/2051] (V3924)

TLE1 Antibody / Transcriptional Corepressor immunohistochemistry analysis of human endometrial carcinoma. Formalin-fixed paraffin-embedded human endometrial carcinoma tissue stained with TLE1 Antibody / Transcriptional Corepressor (clone TLE1/2051) shows strong nuclear HRP-DAB brown staining in tumor epithelial cells, consistent with the nuclear localization of the TLE1 transcriptional corepressor. Transducin-Like Enhancer Protein 1 functions as a Groucho family transcriptional corepressor that regulates gene repression through interactions with transcription factors and chromatin-associated regulatory complexes. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 Tris-EDTA buffer for 10-20 minutes followed by cooling at room temperature prior to immunohistochemical staining.

Western blot testing of human HeLa cell lysate with TLE1 antibody (clone TLE1/2051). Predicted molecular weight ~83 kDa.

TLE1 Antibody / Transcriptional Corepressor protein microarray specificity analysis of clone TLE1/2051. Proteome-scale specificity screening was performed using a HuProt human protein microarray containing more than 19,000 individually purified full-length human proteins. TLE1 Antibody / Transcriptional Corepressor (clone TLE1/2051) demonstrated highly selective binding to Transducin-Like Enhancer Protein 1, producing the strongest signal on the array with a Z-score of 137.39 and an S-score of 120.92. Secondary signals detected for FTL and EEF2K were substantially lower, confirming strong preferential recognition of the intended TLE1 target protein. These data support the high specificity of clone TLE1/2051 for the TLE1 transcriptional corepressor across a large-scale proteomic screening platform.
Z-score represents the strength of the fluorescent signal generated when the antibody binds to a protein on the microarray and is expressed as standard deviations above the mean signal intensity across the array. S-score represents the difference between sequential Z-scores after ranking proteins by signal intensity and therefore reflects the relative specificity of the antibody for its intended antigen compared with other proteins present on the array.

SDS-PAGE analysis of purified, BSA-free TLE1 antibody (clone TLE1/2051) as confirmation of integrity and purity.