TL1A Antibody Recombinant Mouse MAb / TNFSF15 / VEGI [clone rVEGI/1283] (V3637)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant TL1A antibody (clone rVEGI/1283). These results demonstrate the foremost specificity of the rVEGI/1283 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunohistochemistry of TL1A antibody in human spleen tissue. FFPE human spleen shows membranous and cytoplasmic HRP-DAB brown staining predominantly within vascular endothelial structures and scattered stromal cells, consistent with VEGI / TNFSF15 localization in vascular and immune-associated compartments. Clone rVEGI/1283 was used as a recombinant mouse monoclonal antibody for detection. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature for 20 minutes prior to staining.

SDS-PAGE analysis of purified, BSA-free TL1A antibody (clone rVEGI/1283) as confirmation of integrity and purity.